mouse anti-type vii collagen monoclonal antibody  (Cosmo Bio USA)

Journal: PLoS ONE

Article Title: Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers

doi: 10.1371/journal.pone.0145502

Figure Lengend Snippet: Immunohistochemical analysis at the vitreoretinal junction of a paraffin-embedded, serially sectioned (3–4 μ m) human donor eye evaluated by light microscopy. Anti-type VII collagen labeling with monoclonal LH7.2 (sections 1, 3 & 4) and periodic acid-Schiff (sections 2 & 5). Type VII collagen is present in astrocytic corpora amylacea. At least three corpora amylacea were sliced in this series, consecutively visualized in sections 1–2 (white arrows), sections 2–3 (black arrows) and sections 3–5 (black arrowheads). Corpora amylacea reside in the ganglion cell layer and inner plexiform layer (IPL). Type VII collagen is also present in small vesicles, clustered near large nuclei in the inner retina (white arrowhead). The vesicles reside outside the nucleus, and within the cytoplasm (sections 1, 2 & 3 compared). The cytoplasm of the type VII collagen yielding cell type seems more extensive than that of most other cells residing in the ganglion cell layer (GCL). Furthermore, their nuclei are larger and appear less dense in PAS (or hematoxylin) stains. The inner limiting membrane (ILM) does not label visibly for type VII collagen. Negative control section (NC) shows no labeling. (200 x)

Article Snippet: The primary antibodies used were rabbit polyclonal anti-type VII collagen (Calbiochem, Darmstadt, Germany), mouse monoclonal anti-type VII collagen (LH7.2, Abcam, Cambridge, UK; clone 32, Chemicon, Temacula, California, USA), anti-glial fibrillary protein (GFAP) (an astrocyte marker, Sigma), and anti-vimentin (a Müller cell marker, Dako, Glostrup, Denmark) ( ).

Techniques: Immunohistochemical staining, Light Microscopy, Labeling, Negative Control

Journal: PLoS ONE

Article Title: Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers

doi: 10.1371/journal.pone.0145502

Figure Lengend Snippet: Anti-type VII collagen labeling with monoclonal LH7.2. This donor sample contains a lot of cells within the ganglion cell layer (GCL) that have Col VII-positive vesicles in their cytoplasm. Four corpora amylacea reside in the inner plexiform layer (IPL). (400x)

Article Snippet: The primary antibodies used were rabbit polyclonal anti-type VII collagen (Calbiochem, Darmstadt, Germany), mouse monoclonal anti-type VII collagen (LH7.2, Abcam, Cambridge, UK; clone 32, Chemicon, Temacula, California, USA), anti-glial fibrillary protein (GFAP) (an astrocyte marker, Sigma), and anti-vimentin (a Müller cell marker, Dako, Glostrup, Denmark) ( ).

Techniques: Labeling

Journal: PLoS ONE

Article Title: Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers

doi: 10.1371/journal.pone.0145502

Figure Lengend Snippet: Type VII collagen-positive vesicles (LH7.2; green) around two cell nuclei (white arrowheads) in the inner plexiform layer (IPL). Note filamentous GFAP (polyclonal; red) labeling within the nerve fiber layer and around blood vessels. Within the lumen of blood vessels, autofluorescent erythrocytes (*) are seen. They can be discriminated from the corpus amylaceum (white arrow) in terms of fluorescence intensity, size and location. The inner limiting membrane (ILM) directly above the left blood vessel shows some focal positivity for type VII collagen labeling. A control section does not show any Col VII or GFAP labeling. GCL ganglion cell layer. (400x, inset 960x)

Article Snippet: The primary antibodies used were rabbit polyclonal anti-type VII collagen (Calbiochem, Darmstadt, Germany), mouse monoclonal anti-type VII collagen (LH7.2, Abcam, Cambridge, UK; clone 32, Chemicon, Temacula, California, USA), anti-glial fibrillary protein (GFAP) (an astrocyte marker, Sigma), and anti-vimentin (a Müller cell marker, Dako, Glostrup, Denmark) ( ).

Techniques: Labeling, Fluorescence

Journal: PLoS ONE

Article Title: Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers

doi: 10.1371/journal.pone.0145502

Figure Lengend Snippet: Type VII collagen-positive vesicles (LH7.2; green) colocalize with GFAP-positive vesicles (polyclonal; red) around cell nuclei (white arrowheads). In this donor sample, filamentous GFAP labeling is observed in the nerve fiber layer. This overlay projection is of the nerve fiber layer down to the inner IPL. Most Col VII vesicles are located at the junction of ganglion cell layer and inner plexiform layer. Although GFAP positive filaments meander around nuclei, the GFAP vesicles can be distinguished from sectioned filaments. (630x)

Article Snippet: The primary antibodies used were rabbit polyclonal anti-type VII collagen (Calbiochem, Darmstadt, Germany), mouse monoclonal anti-type VII collagen (LH7.2, Abcam, Cambridge, UK; clone 32, Chemicon, Temacula, California, USA), anti-glial fibrillary protein (GFAP) (an astrocyte marker, Sigma), and anti-vimentin (a Müller cell marker, Dako, Glostrup, Denmark) ( ).

Techniques: Labeling

Journal: PLoS ONE

Article Title: Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers

doi: 10.1371/journal.pone.0145502

Figure Lengend Snippet: Image of the inner retina, paraffin section. Type VII collagen labeling (polyclonal; green) colocalizes with that of GFAP (monoclonal; red). The vesicle cluster around one nucleus (left white arrowhead) contains both Col-VII and GFAP-positive vesicles, while another cluster (right white arrowhead) has few Col VII-positive vesicles. Most vesicles contain both type VII collagen and GFAP and label yellowish, but others contain either Col-VII or GFAP. In this donor sample, no filamentous GFAP labeling is detected (by monoclonal antibodies) at the nerve fiber layer (NFL). Two corpora amylacea can be seen, both labeled dually (white arrows). ILM inner limiting membrane, GCL ganglion cell layer, IPL inner plexiform layer, vb vitreous body. (400x)

Article Snippet: The primary antibodies used were rabbit polyclonal anti-type VII collagen (Calbiochem, Darmstadt, Germany), mouse monoclonal anti-type VII collagen (LH7.2, Abcam, Cambridge, UK; clone 32, Chemicon, Temacula, California, USA), anti-glial fibrillary protein (GFAP) (an astrocyte marker, Sigma), and anti-vimentin (a Müller cell marker, Dako, Glostrup, Denmark) ( ).

Techniques: Paraffin Section, Labeling

Journal: PLoS ONE

Article Title: Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers

doi: 10.1371/journal.pone.0145502

Figure Lengend Snippet: Immuno-electron microscopic image of the inner limiting membrane of a human retina. ( A-C ) Immunogold-labeled polyclonal antibody directed against type VII collagen (black arrows) is found in the direct vicinity of vitreous fibrils (white arrows) at the vitreoretinal interface. Some gold labeling is also situated within the inner limiting membrane (ILM). ( A ) Anterior retina, showing sparse, clustered labeling. Vitreous collagen fibrils join the retina. Bar 1 μm. ( B ) The posterior retina has frequent gold labeling at a depth of 150–200 nm in the inner limiting membrane. Bar 500 nm. ( C ) Posterior retina, gold labeling for type VII collagen is mostly at the retinal side of the vitreous fibrils. Müller cell endfeet (M) remain unlabeled. vb vitreous body. ( D ) Negative control of posterior retina shows no labeling. Bar 1μm.

Article Snippet: The primary antibodies used were rabbit polyclonal anti-type VII collagen (Calbiochem, Darmstadt, Germany), mouse monoclonal anti-type VII collagen (LH7.2, Abcam, Cambridge, UK; clone 32, Chemicon, Temacula, California, USA), anti-glial fibrillary protein (GFAP) (an astrocyte marker, Sigma), and anti-vimentin (a Müller cell marker, Dako, Glostrup, Denmark) ( ).

Techniques: Labeling, Negative Control

Journal: PLoS ONE

Article Title: Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers

doi: 10.1371/journal.pone.0145502

Figure Lengend Snippet: Immuno-electron microscopic image of the inner plexiform layer of a human retina. Immunogold-labeled polyclonal antibody directed against type VII collagen labels two corpora amylacea diffusely. Within the corpora amylacea, there is no evidence of fibrillar collagen or banding. Some background labeling is present. Bar 10 μm.

Article Snippet: The primary antibodies used were rabbit polyclonal anti-type VII collagen (Calbiochem, Darmstadt, Germany), mouse monoclonal anti-type VII collagen (LH7.2, Abcam, Cambridge, UK; clone 32, Chemicon, Temacula, California, USA), anti-glial fibrillary protein (GFAP) (an astrocyte marker, Sigma), and anti-vimentin (a Müller cell marker, Dako, Glostrup, Denmark) ( ).

Techniques: Labeling

Journal: PLoS ONE

Article Title: Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers

doi: 10.1371/journal.pone.0145502

Figure Lengend Snippet: Western blot stained for type VII collagen. A 290 kDa band appeared in samples (directly) placed in reducing agent buffer (group/lane A ). Omitting the use of reducing agents resulted in fading of the 290 kDa band (group/lane B ). When samples were incubated in 30 units/ml collagenase, the 290 kDa band disappeared completely, while a 145 kDa band appeared (group/lane C ).

Article Snippet: The primary antibodies used were rabbit polyclonal anti-type VII collagen (Calbiochem, Darmstadt, Germany), mouse monoclonal anti-type VII collagen (LH7.2, Abcam, Cambridge, UK; clone 32, Chemicon, Temacula, California, USA), anti-glial fibrillary protein (GFAP) (an astrocyte marker, Sigma), and anti-vimentin (a Müller cell marker, Dako, Glostrup, Denmark) ( ).

Techniques: Western Blot, Staining, Incubation

Journal: BMC Cancer

Article Title: Collagen mRNA levels changes during colorectal cancer carcinogenesis

doi: 10.1186/1471-2407-9-136

Figure Lengend Snippet: Location of type IV collagen and type VII collagen in normal and colorectal cancer tissue from the same patient . Frozen tissue sections were incubated on the glass slide with polyclonal rabbit anti-type IV collagen and monoclonal mouse anti-type VII collagen. Negative controls without primary antibody were run in parallel (not shown). The bar represents 50 μm.

Article Snippet: The sections were examined by immunofluorescence microscopy for the localization of type IV and type VII collagens using polyclonal rabbit anti type IV collagen (Abcam 6586) and monoclonal mouse anti-type VII collagen (Abcam 6312), respectively.

Techniques: Incubation